Transpiration Investigation

Use a potometer to measure the effect of environmental factors on water uptake.

Procedure

Aim

To investigate the effects of light, humidity, wind, and temperature on the rate of transpiration using a bubble potometer.

Step-by-Step Procedure

  1. 1

    Cut a leafy shoot under water to prevent air bubbles from entering the xylem.

  2. 2

    Insert the shoot into the potometer through a rubber stopper, ensuring an airtight seal with Vaseline.

  3. 3

    Trap an air bubble in the capillary tube by lifting the end out of the water briefly.

  4. 4

    Adjust the environmental conditions (temp, humidity, wind).

  5. 5

    Record the distance moved by the air bubble over a period of 10 minutes.

  6. 6

    Record results for different environmental conditions.